"How do cells navigate their developmental pathways to assume specific fates, and how do their behavior adapt under a range of physiological and pathological conditions?
Our laboratory explores dynamic states of microglia and neurons, particularly in the context of neurodegenerative diseases such as Alzheimer's Disease. We employ data-driven approaches to unravel the complex molecular mechanisms that govern cell state transitions in the brain.
Research Focus
- Decoding Microglial States Across Disease
- Our research delves into the lipid homeostasis and inflammatory responses of microglia in the context of Alzheimer's Disease and the natural aging process. This focus not only promises to enhance our understanding of microglia's role in neurodegenerative disorders but also to pinpoint potential therapeutic targets within their molecular pathways.
- Exploring Neuronal Diversification in the Hypothalamus
- Parallelly, we are investigating the intrinsic and extrinsic mechanisms that drive selective neuronal vulnerability, with a special emphasis on the hypothalamus. Hypothalamus is known as the 'The Heart of The Brain', and regulates homeostatic and innate behaviors.
Methodological Approaches
Approach 1: Computational Dry Lab
- Bioinformatics: Employ single-cell RNA and ATAC analysis, along with spatial transcriptomics.
- Imaging Analysis: Utilize live imaging and multiple single-molecule in situ hybridization techniques
Approach 2: In Vitro Experiments
- Target gene regulatory networks using CRISPR-RNP.
- Implement live imaging and an in-house multiplex single-molecule in situ hybridization method.
Approach 3: In Vivo Studies
- Use transgenic mouse models of Alzheimer's disease.
- Apply viral targeting, single-cell sequencing, and brain clearing with multiplex single-molecule in situ hybridization.
Available Projects
Please contact Thomas via tkim@dandrite.au.dk
