Thomas Kim Lab
 

Project Overview                                

Focus 1: Decoding Microglial States Across Disease

• Specific Focus: Investigate lipid homeostasis and inflammatory responses in microglia in the context of Alzheimer's Disease and Aging.                            

Focus 2: Decoding Neuronal Diversification in the Hypothalamus

• Specific Focus: Identify intrinsic and extrinsic mechanisms driving selective neuronal vulnerability in Alzheimer's Disease and Aging.                            

Focus 3: Turning lead into gold

• Specific Focus: Understand gene regulatory networks driving the onset of neuropeptides and neurotransmitters.                                                  

Methodological Approaches                                   

Approach 1: Computational Dry Lab

• Bioinformatics: Employ single-cell RNA and ATAC analysis, along with spatial transcriptomics
• Imaging Analysis: Utilize live imaging and multiple single-molecule in situ hybridization techniques.                         

Approach 2: In Vitro Experiments

• Target gene regulatory networks using CRISPR-RNP.
• Implement live imaging and an in-house multiplex single-molecule in situ hybridization method.                                          

Approach 3: In Vivo Studies

• Use transgenic mouse models of Alzheimer's disease.
• Apply viral targeting, single-cell sequencing, and brain clearing with multiplex single-molecule in situ hybridization.            
• Conduct behavioral analysis to assess impacts.                          
   

The successfull applicant is:                                                             

Someone who is reasonably motivated but interested in what we do. Also someone who has a good work/life balance.                                

Please contact Group Leader Thomas Kim directly, if interested.