Example of a current/available student project (Bachelor/Master/PhD)
Vision restoration by in vivo direct reprogramming for converting glial cells to neurons
We aim to reprogram retinal glial cells to convert to neurons in vivo to restore vision in retinal neurodegeneration such as glaucoma. For this purpose we will transfer genetic and epigenetic factors to genetically labeled retinal glial cells by the injection of adeno-associated viral vectors.
Lab background:
We address these fundamental questions in circuit neuroscience mainly by focusing on visual circuits across retina, superior colliculus, thalamus and visual cortex. The logic of our research plan is to first identify a computation performed by a given neuronal circuit comprising distinct cell types in the adult brain. Second, to investigate how the computation is performed by linking the activity and synaptic connectivity of individual cell types in the circuit to the computation that the circuit achieves. Third, to examine the role of individual cell types in transforming the sensory input into output behavior. Finally, to study the genetic mechanisms by which the elementary circuit motifs are assembled, and how its dysfunction can lead to disease.
Methods:
In vivo 2-photon functional imaging, electrophysiology, optogenetics, trans-synaptic viral tracing, behavioral analysis, molecular biology, transcriptomics, proteomics, and mouse genetics.
Other student (master/PhD) projects available:
Brain circuit mapping using novel light-inducible recombinase systems
Imaging of brain activity in freely-moving mice using head-mount miniscope
Single-cell transcriptome analysis of mouse superior colliculus for characterizing cell types
In vivo genome editing for visualizing endogenous neuronal proteins
Development of a viral vector-based cell type targeting method for gene therapy
The successful applicant is:
Hard working, team working, curiosity driven, ambitious, analytical, good at programming
Please contact Group Leader Keisuke Yonehara directly, if interested.
