Study opportunities

Mark Denham's lab

Lab background:

The Denham group works with iPSCs to study how the nervous system develops and the processes involved in neuro-degeneration. In particular, the lab has reprogrammed fibroblasts from patients with familial Parkinson’s diseased mutations into iPSCs and uses these to investigate genetic mechanisms that influence Parkinson’s disease. The lab has developed efficient protocols for generating neurons from the iPSC lines and has used RNA sequencing to identify candidate genes that may be involved in the early stages. Our research goals are to further investigate these gene candidates to identify the affected signaling pathways.

Available projects:

Investigating genetic causes of Parkinson’s disease using stem cell derived neurons.

Patients that are heterozygous carriers for mutations in the GBA gene are predisposed to developing Parkinson’s disease. Our lab had derived induced pluripotent stem cells (iPSCs) from GBA heterozygous patients and we are using these to investigate the genetic mechanisms that occur during the early stages of Parkinson’s disease.

Methods:

Induced pluripotent stem cell culture, differentiation of IPSCs into neurons, Immunofluorescence, lentiviral delivery of transgenes or CRISPR constructs, multi-electrode array analysis of neuronal activity.

Please contact Group Leader Mark Denham directly, if interested.