Our activities are distributed on three areas
1. DAGMAR, generation of genetically modified mice.
Since the advent of CRISPR technology, many gene modifications can be directly done in zygotes, eliminating the need of generating mice from ES cells by chimeras. However, more complex alterations like the exchange of larger DNA sequences (e.g. mouse to human) still are best done in ES cells. ES cells are also a valuable tool to study the genetic influence on cellular differentiation.
In addition to our own projects, we offer collaborations for the generation of genetically modified mice and murine ES cells. This includes, but is not limited to:
As a service we also offer cryopreservation, rederivation and sanitation of mouse lines.
2. Translational regulation of the bHLH transcription factor TWIST1
We have earlier observed that translation of the bHLH transcription factor TWIST1 is differentially regulated in the developing embryo. We identified a number of micro-RNAs that inhibit translation in cultured cells. Using CRISPR based techniques, we try to identify regulatory elements that are responsible for the translational inhibition in the embryo.
3. Functional analysis of SEPTIN9 isoforms
Septins are a family of filament forming proteins. Septin filaments, which are referred to as the fourth part of the cytoskeleton, consist of repeats of heteromeric octamers. Several Septins, are associated with a variety of tumors. This is particularly true for Septin9, which surprisingly seem to have both proto-oncogenic and tumor suppressor function. This dualistic function might be connected to different SEPTIN9 isoforms that have been shown to differentially direct the association of Septin filaments with microtubules and acting filaments.
We try to elucidate the impact of SEPTIN9 isoforms on the cellular localization and cell stability.