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Study opportunities

Keisuke Yonehara's lab


Example of a current/available student project (Bachelor/Master/PhD)
Brain circuit mapping using light-inducible recombinase systems

In this project we will modify genomic manipulation enzymes, making them light inducible: this will enable high-throughput, single-cell genomic modification in response to brief pulses of light in the brain. We will use this method to study the connections, morphology, function, and genetic identify of individual neurons within the brain.  

Lab background:

·        How are sensory inputs processed by visual circuits?

·        What is the function of individual cell types in computation and behaviour?

·        What are the genetic and activity-dependent mechanisms of circuit development?

We address these questions mainly by focusing on visual circuits across retina, superior colliculus, thalamus and visual cortex. The logic of our research plan is to first identify a computation performed by a given neuronal circuit comprising distinct cell types in the adult brain. Second, to investigate how the computation is performed by linking the activity and synaptic connectivity of individual cell types in the circuit to the computation that the circuit achieves. Third, to examine the role of individual cell types in transforming the sensory input into output behavior. Finally, to study the genetic mechanisms by which the elementary circuit motifs are assembled, and how its dysfunction can lead to disease.

Methods:
In vivo 2-photon functional imaging, electrophysiology, optogenetics, trans-synaptic viral tracing, behavioral analysis, molecular biology, genomics, and mouse genetics.

Other student (master/PhD) projects available:
Single-cell transcriptome analysis of mouse superior colliculus for characterizing cell types.

The successful applicant is:
Hard working, team working, curiosity driven, ambitious, analytical, good at programming.

Please contact Group Leader Keisuke Yonehara directly, if interested.